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嗜热乙醇杆菌中醇脱氢酶E的功能与调控
  • 摘要

    对嗜热乙醇杆菌中的醇脱氢酶E(alcohol dehydrogenase E, AdhE)进行了克隆、表达和鉴定,证明先前报道的醛脱氢酶其实是AdhE.对AdhE在乙醇代谢中的功能与调控进行了研究.通过分析测定AdhE的酶学性质、AdhE在嗜热乙醇杆菌的不同生长时期的表达情况以及AdhE高表达时嗜热乙醇杆菌产乙醇/乙酸的比率变化,证明了AdhE的主要生理功能是促使乙醇产生.分析了嗜热乙醇杆菌在山梨糖(强还原性碳源)、葡萄糖(氧化还原性居中的碳源)和葡糖醛酸(强氧化性碳源)诱导培养下的AdhE表达量,证明氧化还原力是AdhE的表达调控信号之一.

  • 作者

    彭惠  毛忠贵  武国干  邵蔚蓝  PENG Hui  MAO Zhong-gui  Wu Guo-gan  SHAO Wei-lan 

  • 作者单位

    食品科学与技术国家重点实验室,江南大学,江苏,无锡,214122/南京师范大学,微生物工程重点实验室,江苏,南京,210097/食品科学与技术国家重点实验室,江南大学,江苏,无锡,214122;南京师范大学,微生物工程重点实验室,江苏,南京,210097

  • 刊期

    2008年2期 ISTIC PKU

  • 关键词

    嗜热乙醇杆菌  醇脱氢酶E  功能  调控 

参考文献
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