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褐菖鲉鳃丝EROD生物标志物监测技术的研究与应用
  • 摘要

    以褐菖鲉为实验鱼类,以其鳃丝EROD为研究对象,进行了室内鳃丝EROD活性测定方法条件的摸索和现场海域石油类污染监测的初步应用研究.室内EROD活性测定条件摸索实验结果表明:褐菖鲉鳃丝EROD活性测定的最适pH值为7.8、最适温度为35℃、最适鳃丝用量为18.8±1.9 mm;在鳃丝总长相近(约20 mm)的条件下,剪切成2 mm长的鳃丝片段与否对于EROD活性无显著差异;反应50 min内EROD具有较高活性,随后呈现下降趋势.2次厦门西海域污染现场监测结果表明:褐菖鲉鳃丝EROD活性与海水石油类含量具有良好的正相关趋势,与对照组比较总体上具有显著的差异.因此,褐菖鲉鳃丝的EROD活性测定方法与褐菖鲉肝的一样,能够作为生物标志物应用于现场海区的石油类污染及其生化效应的监测.

  • 作者

    陈清福  张玉生  郑榕辉  CHEN Qing-fu  ZHANG Yu-sheng  ZHENG Rong-hui 

  • 作者单位

    国家海洋局第三海洋研究所,福建,厦门,361005

  • 刊期

    2010年3期 ISTIC PKU

  • 关键词

    海洋生物学  褐菖鲉  鳃丝EROD  生物标志物  石油类  方法  监测 

参考文献
  • [1] 沈南南,李纯厚,王晓伟. 石油污染对海洋浮游生物的影响. 生物技术通报, 2006,z1
  • [2] 郭志平. 我国近海面临的石油污染及其防治. 浙江海洋学院学报(自然科学版), 2004,3
  • [3] 陈建秋. 中国近海石油污染现状、影响和防治. 节能与环保, 2002,3
  • [4] J(o)nsson E M;Abrahamson A;Brunstr(o)m B. Cytochrome P4501A induction in rainbow trout gills and liver following exposure to waterborne indigo,benzo[a]pyrene and 3,3,4,4,5-pentachlorobiphenyl. Aquatic Toxicology, 2006
  • [5] Burke M D;Mayer R T. Ethoxyresorufin:direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metabolism and Disposition, 1974
  • [6] UNEP. Earth watch:oil pollution,chemical pollution--a global review. New York:UNEP, 1992
  • [7] EPA. Water quality criteria documents. Washington:Environmental Protection Agency, 1979
  • [8] 周文敏;傅德黔;孙宗光. 水中优先控制污染物黑名单. 中国环境监测, 1990,04
  • [9] Payne J F;Penrose W R. Induction of ary1 hydrocarbon(benzo(a)pyrene) hydroxylase in fish by petroleum. Bull Environ Cont Toxieol, 1975
  • [10] Payne J F. Field evaluation of benzopyrene hydroxylase induction as a monitor for marine pollution. Science, 1976
  • [11] Abrahamson A;Andersson C;J(o)nsson E M. Gill EROD in monitoring of CYP1A inducers in fish-A study in rainbow trout (Oncorhynchus mykiss) caged in Stockholm and Uppsala waters. Aquatic Toxicology, 2007
  • [12] J(o)nsson E M;Abrahamson A;Brunstr(o)m B. EROD activity in gill filaments of anadromous and marine fish as a biomarker of dioxin-like pollutants. Comparative Biochemistry and Physiology Part C:Toxicology and Pharmacology, 2003
  • [13] Abrahamson A. Gill EROD activity in fish. Uppsala:Uppsala University, 2007
  • [14] J(o)nsson E M. A gill filament EROD assay. Uppsala:Evolutionary Biology Centre of Uppsala University, 2003
  • [15] 国家海洋局. GB 3097-1997,海水水质标准. 北京:中国标准出版社, 1997
  • [16] 国家海洋局. 2008年中国海洋环境质量公报. 北京:海洋出版社, 2009
  • [17] Otte J C;Andersson C;Abrahamson A. A bioassay approach to determine the dioxin-like activity in sediment extracts from the Danube River:Ethoxyresorufin-O-deethylase induction in gill filaments and liver of three-spined sticklebacks (Gasterosteus aculeatus). Environmental International, 2008
  • [18] Abrahamson A;Brandt I;Brunstr(o)m B. Monitoring contaminants from oil production at sea by measuring gill EROD activity in Atlantic cod (Gadus morhua). Environmental Pollution, 2008
  • [19] 吴常文;王志铮;王伟洪. 褐菖鲉Sebastiscus marmoratus增养殖技术研究. 浙江水产学院学报, 1997,03
  • [20] J(o)nsson E M;Brandt I;Brunstr(o)m B. Gill filament-based EROD assay for monitoring waterborne dioxin-like pollutants in fish. Environmental Science and Technology, 2002
  • [21] 国家海洋局. GB 17378.4-2007,海洋监测规范第4部分:海水分析. 北京:中国标准出版社, 2007
  • [22] Andersson C;Katsiadaki I;Lundstedt-Enkel K. Effects of 17α-ethynylestradiol on EROD activity,spiggin and vitellogenin in three-spined stickleback (Gasterosteus aculeatus). Aquatic Toxicology, 2007
  • [23] Garrigues P. Final report BEEP project. Belgi:BEEP, 2004
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