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内蒙古白绒山羊毛囊发育周期蛋白质表达谱分析
  • 摘要

    山羊绒毛的产量和绒毛纤维的质量与角蛋白有关.本研究旨在探讨内蒙古白绒山羊(Capra cashmere)毛囊差异表达蛋白质的变化规律,采用双向凝胶电泳(two dimensional gel electrophoresis,2-DE)技术分离一年中12个月份毛囊总蛋白质,建立了毛囊发育周期蛋白质图谱.使用质谱鉴定差异表达蛋白点,并分析部分蛋白点的表达趋势.结果表明,12个月样本的2-DE图谱显示,平均每块胶上可检测到255个蛋白点,分布在pI 4~7,分子量30~85 kD;软件分析得到20个差异表达蛋白点,质谱成功鉴定出12种蛋白质;这些蛋白质主要为角蛋白(keratin,K),参与细胞生物过程并发挥生物学功能.将12个月做以下划分:11、12、1和2月4个月归为毛囊退行期,3和4月归为毛囊休止期,此时蛋白质表达量明显降低;5、6、7、8、9和10月归为毛囊兴盛期,蛋白质表达量为一年中最高.初步得出K1、K5、K71和K25在毛囊发育的兴盛期表达量高,可能促进毛囊的生长发育.K83和K10在毛囊休止期表达量高,可能与毛囊的休止有关.研究建立了良好的皮肤毛囊蛋白质表达谱,分析并鉴定了差异蛋白质及其表达趋势,对于从蛋白质组学研究内蒙古白绒山羊毛囊生长和发育,寻找标记蛋白应用于绒山羊育种提供了新的研究线索.

  • 作者

    高丽霞  张燕军  张文广  王志新  奈日乐  杜琛  王乐  刘志红  李金泉 

  • 作者单位

    内蒙古农业大学动物科学学院/动物遗传育种与繁殖自治区重点实验室,呼和浩特010018;包头轻工职业技术学院乳品工程学院,包头014035/内蒙古农业大学动物科学学院/动物遗传育种与繁殖自治区重点实验室,呼和浩特,010018

  • 刊期

    2014年6期 ISTIC PKU

  • 关键词

    白绒山羊  毛囊  差异蛋白质  双向电泳  质谱鉴定  Cashmere goat  Hair follicle  Differential proteomics  Two-dimensional gel electrophoresis  Mass spectrometry identification 

参考文献
  • [1] 冯静,杨华,宋天增,杨剑波,石国庆,徐从祥,黄锡霞. 绵羊KAP1.1基因克隆与单核苷酸多态性分析. 西南农业学报, 2010,5
  • [2] 刘海英,杨桂芹,张微,朱晓萍,贾志海. FGF5基因对内蒙古绒山羊绒毛性状的影响. 遗传, 2009,2
  • [3] 田月珍,黄锡霞,柏妍,田可川,徐新明,吴伟伟,哈尼克孜. 与毛囊生长发育及羊毛、羊绒品质相关基因的研究进展. 中国草食动物科学, 2013,3
  • [4] 松杰,董扬,孟丽云,杜琛,苏蕊,李金泉,张文广. 内蒙古绒山羊皮肤蛋白质双向电泳条件优化及图谱建立. 中国畜牧兽医, 2012,7
  • [5] 余娟娟,刘积凤,赵金山,程明,刘开东,柳楠. 敖汉细毛羊Ⅰ型内根鞘角蛋白基因表达模式的研究分析. 安徽农业科学, 2012,18
  • [6] Coulombe P A;Omary M B. Hard' and 'soft' principles defining the structure,function and regulation of keratin intermediate filaments. Current Opinion in Cell Biology, 2002,01
  • [7] Fang Y;Liu W J;Zhang F Q. The polymorphism of a novel mutation of KAPI3,1 gene and its associations with cashmere traits on Xinjiang local goat breed in China. Asian Journal of Animal and Ventrinary Advances, 2010,01
  • [8] Fang Y;Song F F;Zhang L. Differential antennal proteome comparison of adult honeybee drone,worker and queen(Apis mellifera L.). Journal of Proteomics, 2012,03
  • [9] Kartasova T;Roop D R;Holbrook K A. Mouse differentiation-specific keratins 1 and 10 require a preexisting keratin scaffold to form a filament network. The Journal of Cell Biology, 1993,05
  • [10] Kopan R;Fuchs E. A new look into an old problem:Keratins as tools to investigate determination,morphogenesis,and differentiation in skin. Genes and development, 1989,01
  • [11] Koch P J;Roop D R. The role ofkeratins in epidermal development and homeostasis-going beyond the obvious. The Journal of Investigative Dermatology, 2004,05
  • [12] Langbein L;Rogers M A;Praetzel-Wunder S. K25(K25irs1),K26(K25irs2),K27(K25irs3),and K28(K25irs4)represent the type Ⅰ inner root sheath keratins of the human hair follicle. Joumal of Investigative Dermatology, 2006,11
  • [13] Liu W J;Fang Y;Zhang L P. The polymorphism of a mutation of KAP16.6 gene on three goat breeds in China. Journal of Animal and Veterinary Advances, 2009,12
  • [14] Moll R;Franke W W;Schiller D L. The catalog of human cytokeratins:Patterns of expression in normal epithelia,tumors and cultured cells. CELL, 1982,01
  • [15] Moll I;Heid H;Franke W W. Distribution of a special subset of keratinocytes characterized by the expression of cytokeratin 9 in adult and fetal human epidermis of various body sites. DIFFERENTIATION, 1987,03
  • [16] Peters T;Sedlmeier R;Buessow H. Alopecia in a novel mouse model RCO3 is caused by mK6irsl deficiency. Journal of Investigative Dermatology, 2003,04
  • [17] Porter R M;Gandi M;Wilson N J. Functional analysis of keratin components in the mouse hair follicle inner root sheath. The British Journal of Dermatology, 2004,02
  • [18] Szeverenyi I;Cassidy A J;Chung C W. The human intermediate filament database:Comprehensive information on a gene family involved in many human diseases. Human Mutation, 2008,03
  • [19] Thummel R;Bai S;Sarras M P J. Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfrl and msxb. DEVELOPMENTAL DYNAMICS, 2006,02
  • [20] Ullrich R;Paus R. Molecular principles of hair follicle:Induction and morphogenesis. BIOESSAYS, 2005,03
  • [21] Aya K;Kosuke T;Yuki Y. Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal. Cellular Signalling, 2007,12
  • [22] Bawden C S;McLaughlan C;Nesci A. A unique type Ⅰ keratin intermediate filament gene family is abundantly expressed in the inner root sheaths of sheep and human hair follicles. Journal of Investigative Dermatology, 2001,01
  • [23] Cockett N E;Shay T L;Smit M. Analysis of the sheep genome. Physiological Genomies, 2001,02
  • [24] Cooper D,Sun T. Monoclonal antibody analysis of bovine epithelial keratins.Specific pairs as defined by coexpression. The Journal of Biological Chemistry, 1986,10
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