登录 | 注册 | 充值 | 退出 | 公司首页 | 繁体中文 | 满意度调查
综合馆
马立克氏病病毒CVI988株病毒囊膜糖蛋白B启动子的克隆及活性分析
  • 摘要

    为了选择适宜的启动子调控外源基因的表达,以改善马立克氏病毒对载体的重组病毒的免疫保护力,克隆了马立克氏病病毒(MDV)CVI988株病毒囊膜糖蛋白B(gB)启动子,通过测序分析发现克隆的gB启动子与GenBank上发表的MDV GA株序列的同源性为99.5%.分别以β-半乳糖苷酶(β-galactosidase)和虫荧光素酶(luciferase)为报告基因,构建pSK-gB-LacZ和pgB-Luc真核表达载体.将pSK-gB-LacZ转染的中国地鼠卵巢(Chinese hamster ovary,CHO)细胞进行染色,出现蓝色,说明在CHO细胞中,MDVgB启动子可以有效地调控lacZ基因的转录.在CEF细胞中,利用虫荧光素酶系统将gB启动子与SV40及hCMV启动子进行活性比较,发现hCMV启动子的活性为gB启动子的31倍,SV40启动子为gB启动子的27倍.

  • 作者

    邱亚峰  葛菲菲  徐学清  陈溥言  QIU Ya-feng  GE Fei-fei  XU Xue-qing  CHEN Pu-yan 

  • 作者单位

    南京农业大学动物疫病诊断与免疫重点实验室,南京,210095

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    马立克氏病毒  启动子  转录  转染 

参考文献
  • [1] Sonoda K;Sakaguchi M;Okamura H;Yokogawa K;Tokunaga E;Tokiyoshi S;Kawaguchi Y;Hirai K. Development of an effective polyvalent vaccine against both Marek's and Newcastle diseases based on recombinant Marek's disease virus type 1 in commercial chickens with maternal antibodies.. Journal of Virology, 2000,7
  • [2] Masashi S;Hideki N;Kengo S. Protection of chickens with or without maternal antibodies against both Marek's and Newcasle diseases by one-time vacation with recombinant vaccine of Marek's disease virus type 1. Vaccine, 1998,16
  • [3] Ross L J N;Sanderson M;Scott S D. Nucleotide sequence and characterization of the Marek's disease virus homologue of glycoprotein B of Herpes simplex virus. Journal of General Virology, 1989
  • [4] Sambrook J;Russell D W. Molecular Cloning:A Laboratory Manual. New York,Cold Spring Harbor Laboratory Press, 2001
  • [5] Messerle M;Keil G M;Schneider K;Koszinowski U. Characterization of the murine cytomegalovirus genes encoding the major DNA binding protein and the ICP18.5 homolog. Virology, 1992
  • [6] Mettenleiter T C;Saalmuller A;Weiland F. Pseudorabies virus protein homologues to Herpes simplex virus type 1 ICP18.5 is necessary for capsid maturation. Journal of Virology, 1993,03
  • [7] Robin W M;John L C;Caroline H M. Transfection of chicken embryo fibroblasts with Marek's diseas virus DNA. Avian Diseases, 1990
  • [8] Kenji T;Chiaki Y;Nobuhiko T. Protection of chickens against very virulent Infectious bursal disease virus (IBDV)and Marek's disease virus (MDV) with a recombinant MDV expressiong IBDV VP2. Virology, 1999
  • [9] Beuken E R;Slobbe R;Bruggeman C;AVink C. Cloning and sequence analysis of the genes encoding DNA polymerase,glycoprotein B,ICP1 8.5 and major DNA-binding protein of rat cytomegalovirus. Journal of General Virology, 1996
查看更多︾
相似文献 查看更多>>
52.206.226.77