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黑曲霉木聚糖酶基因xynB在大肠杆菌中的表达及重组木聚糖酶的特性
  • 摘要

    从黑曲霉(Aspergillus niger)mafic-005中克隆得到木聚糖酶基因xynB.序列分析表明,该基因全长745 bp,含有一个67bp内含子,编码225个氨基酸,理论分子量为24 kD(GenBank登录号:DQ174549),与已知黑曲霉xynB基因的同源性均较高.将该基因定向插入大肠杆菌(Escherichia coli)表达载体pET-28a(+)上,并转化E coliBL21获得重组菌株.经过IPTG诱导,xynB基因获得特异性表达.经SDS-PAGE分析,重组蛋白分子量约为30 kD.该重组蛋白经镍NTA琼脂糖凝胶FF纯化后达到电泳纯.酶学性质分析表明,重组木聚糖酶最适温度为40℃,最适pH值为5.0,在酸性和常温条件下具有良好的稳定性.

  • 作者

    邓萍  曹云鹤  陆文清  郭小华  DENG Ping  CAO Yun-he  LU Wen-qing  GUO Xiao-hua 

  • 作者单位

    中国农业大学动物营养国家重点实验室,北京,100094

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    黑曲霉  木聚糖酶  xynB基因  原核表达 

参考文献
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