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稻瘟菌无毒基因簇的RAPD标记及其SCAR转化
  • 摘要

    为克隆稻瘟菌无毒基因簇Avr-Pi1、Avr-Pi2和Avr-Pi4a,以RAPD方法对稻瘟菌(Magnaporthe grisea)菌株FJ81278ZB15、GUY11及其F1后代群体进行扩增,得到2个与该无毒基因簇连锁的分子标记P1414700和P1389420.对2个特异片段进行克隆和测序,并根据序列分别设计2对特异引物,对菌株FJ81278ZB15、GUY11及其F1后代群体进行扩增,扩增结果P1414700成功转化为SCAR标记SC1414,而P1389420未能成功转化.对分子标记SC1414、P1389420和报道的RPF1.2与无毒基因簇Avr-Pi1、Avr-Pi2和Avr-Pi4a遗传连锁关系进行了分析,结果SC1414、RPF1.2、P1389420与Avr-Pi2的遗传距离分别为5.8、2.2和4.4 cM;与Avr-Pi1的遗传距离分别为15.9、7.9和5.7 cM;与Avr-Pi4a的遗传距离分别为20.7、12.7和10.5 cM.

  • 作者

    林艳  王宝华  杨婕  雷财林  鲁国东  王宗华  LIN Yan  WANG Bao-hua  YANG Jie  LEI Cai-lin  LU Guo-dong  WANG Zong-hua 

  • 作者单位

    福建农林大学生物农药与化学生物学教育部重点实验室,功能基因组学研究中心,福州,350002/中国农业科学院重大工程中心,北京,100080

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    稻瘟菌  无毒基因簇  分子标记 

参考文献
  • [1] 何月秋. 真菌菌丝体培养和提取DNA方法的改进. 菌物系统, 2000,3
  • [2] 王宝华,鲁国东,林伟明,王宗华. 稻瘟病菌无毒基因Avr-Pi1、Avr-Pi2和 Avr-Pi4a的遗传分析及其分子标记. 遗传学报, 2002,9
  • [3] 张国珍,刘俊峰,马秋娟,彭友良. 一个新的与稻瘟病菌无毒基因AVR-Pikm紧密连锁的SCAR标记. 植物病理学报, 2004,6
  • [4] 刘俊峰,张国珍,马秋娟,彭友良. 一个与稻瘟病菌无毒基因AVR-Pikm连锁的SCAR标记的分离. 植物病理学报, 2003,2
  • [5] 张连洪,燕继晔,赵文生,张国珍,国立耘,彭友良. 稻瘟菌无毒基因AVR-Pikm的定位. 植物病理学报, 2006,2
  • [6] 李成云,罗朝喜,李进斌,沈瑛,伊势一男. 稻瘟病菌无毒基因的分子标记. 中国农业科学, 2000,3
  • [7] Heidi U. Bohnert;Isabelle Fudal;Waly Dioh;Didier Tharreau;Jean-Loup Notteghem;Marc-Henri Lebrun. A Putative Polyketide Synthase/Peptide Synthetase from Magnaporthe grisea Signals Pathogen Attack to Resistant Rice. The Plant Cell, 2004,9
  • [8] Ou S H. Rice Diseases. Surrey UK:Commonwealth Mycological Institute, 1985
  • [9] Orbach M J;Farrall L;Sweigard J A;Chumley F G,Valent B. A telomeic avirulence gene determines efficacy for the rice blast resistance gene Pi-ta. The Plant Cell, 2000
  • [10] Paran I;Michelmore R W. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics, 1993
  • [11] Silue D;Notteghem J L;Tharreau D. Evidence of a gene-for gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem. Phytopathology, 1992,05
  • [12] Dean R A;Talbot N J;Ebbole D J;Farman M L Mitchell T K. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 2005
  • [13] Swegard J A;Carroll A M;Kang S;Farrall L Chumley F G,Valent B. Identification,cloning,and characterization of PWL2,a gene for host species specificity in the rice blast fugus. The Plant Cell, 1995
  • [14] Valent B;Farrall L;Chumley F G. Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics, 1991,01
  • [15] 张学博;余菊生;唐乐尘;鲁国东 林成辉. On the resistance classification of indica rice varieties to blast. 植物病理学报, 1994,04
  • [16] Dioh W;Tharreau D;Notteghem J L;Orbach M,Lebrun M H. Mapping of avirulence genes in the rice blast fungus Magnaporthe grisea with RFLP and RAPD markers. Molecular Plant-Microbe Interactions, 2000
  • [17] Zhu H;Blackmon B P;Sasinowski M;Dean R A. Physical map and organization of chromosome 7 in the rice blast fungus,Magnaporthe grisea. Genome Research, ,08
  • [18] Farman M L;Leong S A. Chronosome walking to AVR1-CO39 avirulence gene of Magnaporthe grisea:Discrepancy between the physical and genetic maps. Genetics, 1998
  • [19] Kang S;Sweigard J A;Valent B. The PWL host specificity gene family in the blast fungus Magnaporthe grisea. Molecular Plant-Microbe Interactions, 1995
  • [20] Notteghem J L;Thatteau D;Silue D;Roman D. Present knowledge on rice resistance genetics and strategies for analycis of M.grisea pathogenicity and avirulence genes. U K:CAB Int.Wallingfond, 1994
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