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Erwinia chrysanthemi分离株CSCL006 hrpN基因的克隆与高效表达
  • 摘要

    通过构建Erwinia chrysanthemi分离株CSCL006的DNA文库,克隆出hrpN CSCL006基因,测序结果显示该基因编码区长1 020 bp;推导的harpinCSCL006与Erwinia chrysanthemi ECl6和3937编码的harpin蛋白同源性高,但与其它软腐菌的harpin蛋白同源性较低;在大肠杆菌中(Escherichia coli)高效表达了hrpN CSCL006基因,重组harpinCSCL006蛋白分子量为34kD.以抗harpinEOC的抗体为探针,Western blot证实该蛋白确为harpin;纯化的harpinCSCL006,能引起烟叶的过敏反应.

  • 作者

    汤承  崔亚亚  吴伯骥  李名扬  TANG Cheng  CUI Ya-ya  WU Bo-ji  LI Ming-yang 

  • 作者单位

    西南大学园艺园林学院,重庆,400715;西南民族大学生命科学与技术学院,成都,610041/中国科学院成都生物研究所,成都,610041/西南大学园艺园林学院,重庆,400715

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    Erwinia chrysanthemi  hrpN基因  harpin蛋白  过敏反应 

参考文献
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