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三重巢式PCR技术检测抗草甘膦转基因大豆深加工产品
  • 摘要

    实验以7种具有代表性的含有大豆成分的深加工产品(卵磷脂、大豆蛋白质粉、巧克力饮品、婴儿米粉、大豆粗油、大豆精炼油和大豆色拉油)作为样品进行定性检测,第一轮三重PCR均未能扩增出任何大豆成分,其灵敏度为0.5%.第二轮三重PCR可以扩增出除大豆精炼油和色拉油以外的所有深加工产品的内源基因Lectin、35S-CTP和EPSPS-NOS基因,其检测灵敏度达到0.005%,结果提示三重巢式PCR检测方法适用于大豆深加工产品的定性检测.

  • 作者

    张明辉  高学军  于艳波  秦君  李庆章  ZHANG Ming-hui  GAO Xue-jun  YU Yan-bo  QIN Jun  LI Qing-zhang 

  • 作者单位

    东北农业大学生命科学与生物技术研究中心,黑龙江省农业生物基因检测中心,哈尔滨,150030

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    抗草苷膦转基因大豆  深加工产品  三重巢式PCR  转基因检测 

参考文献
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