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抗苯巴比妥单克隆抗体杂交瘤细胞株的筛选及ciELISA试剂盒的研制
  • 摘要

    用BSA-pAPB免疫Balb/c小鼠,用细胞融合技术制备并用间接ELISA和阻断ELISA筛选抗苯巴比妥单克隆抗体(PB mAb)杂交瘤细胞株,体内诱生腹水法生产PB mAb,应用PB mAb研制PB残留竞争ELISA(ciELISA)快速检测试剂盒(PB-Kit),并测定其性能.结果表明,筛选出3株杂交瘤细胞,最好的3F6-C4株的PB mAb间接ELISA效价为1∶6.4×105,亲和常数(Ka)为1.96×1010 L/moL,半数抑制浓度(IC50)为5.7μg/L,与巴比妥的交叉反应率(CR%)12.4%,与其它化合物无CR;PB-Kit的线性检测范围1.0~81μg/L,灵敏度0.75μg/L,检测限1μg/L;饲料样和猪尿样的平均添加回收率85.8%和91.3%,平均批内和批间变异系数均<15%.PB-Kit具有快速、敏感、特异、简便等特点,适合PB残留快速检测的推广应用.

  • 作者

    王自良  张改平  张海棠  杨艳艳  王艳荣  柴书军  WANG Zi-liang  ZHANG Gai-ping  ZHANG Hai-tang  YANG Yan-yan  WANG Yan-rong  CHAI Shu-jun 

  • 作者单位

    河南科技学院动物科学学院,新乡,453003/河南省动物免疫学重点实验室,郑州,450002

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    苯巴比妥  杂交瘤细胞  单克隆抗体  ciELISA  快速检测试剂盒 

参考文献
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