登录 | 注册 | 充值 | 退出 | 公司首页 | 繁体中文 | 满意度调查
综合馆
CpG-DNA对大肠杆菌诱导的大鼠实验性乳腺炎的影响
  • 摘要

    72只雌性大鼠随机分成对照组和实验组(n=36).对照组产后0 h于左后腿胫骨前肌肌肉注射0.01 mol/L,pH 7.2磷酸盐缓冲液(PBS)100μL,实验组肌注CpG-DNA 200μg/只,72 h后经乳头管灌注2×1012cfu(colony forming unit/mL)/mL,大肠杆菌(Escherichia coli)100 μL/侧到第4对(两侧)乳腺内,分别于灌注前0 h,灌注后8,16,24,48和72 h(n=6)颈静脉放血处死.组织学观察显示大鼠感染后16 h为乳腺腺泡嗜中性粒细胞(polymorphonuclear leukocytes,PMN)浸润高峰,实验组PMN浸润迅速,72 h已无浸润.乳腺组织细菌数在16 h上升至最高,实验组大鼠感染后16、24和48 h乳腺组织细菌数较对照组有显著下降.CpG-DNA能极显著地提高感染前乳腺组织中IL-2水平.乳腺组织IL-6在不同时间点均有显著上升,在16 h达最高.CpG-DNA能显著地提高16 h乳腺组织IL-6水平.实验组乳腺组织N-acetyl-β-D-glucosaminidase(NAGase)无显著变化.表明CpG-DNA可加速炎症初期PMN的快速浸润,促进IL-2和IL-6的产生,减少细菌数量,减轻炎症介质对细胞的损伤并缩短炎症过程,提示CpG-DNA对大肠杆菌诱发的大鼠实验性乳腺炎有一定的预防作用.

  • 作者

    朱于敏  马海田  陈伟华  邹思湘  ZHU Yu-min  MA Hai-tian  CHEN Wei-hua  ZOU Si-xiang 

  • 作者单位

    南京农业大学农业部动物生理生化重点开放实验室,南京,210095

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    CpG-DNA  乳腺炎  大鼠  大肠杆菌 

参考文献
  • [1] 钟凯,王艳玲,邹思湘,陈伟华. 乳头管灌注内毒素诱发大鼠实验性乳腺炎模型的建立. 农业生物技术学报, 2005,5
  • [2] Bannerman DD;Paape MJ;Lee JW;Zhao X;Hope JC;Rainard P. Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.. Clinical and diagnostic laboratory immunology, 2004,3
  • [3] Rambeaud M;Almeida RA;Pighetti GM;Oliver SP. Dynamics of leukocytes and cytokines during experimentally induced Streptococcus uberis mastitis. Veterinary immunology and immunopathology, 2003,3/4
  • [4] Haiqi H E;Virginia K Lowry;Christina L. In vitro activation of chicken leukocytes and in vivo protection against Salmonella enteritidis organ invasion and peritoneal S.enteritidis infection-induced mortality in neonatal chickens by immunostimulatory CpG oligodeoxynucleotide. FEMS Immunology and Medical Microbiology, 2005,43
  • [5] Hartmann G;Krieg A M. Mechanism and function of a newly identified CpG DNA motif in human primary B cells. Journal of Immunology, 2000,02
  • [6] Kei Yasuda;Yoshiyuki Ogawa;Michiyuki Kishimoto. Plasmid DNA activates murine macrophages to induce inflammatory cytokines in a CpG motif-independent manner by complex formation with cationic liposomes. Biochemical and Biophysical Research Communications, 2002,293
  • [7] Kirk R Schultz;Winnie N S U;Hsiao Chih-Cheng. Chloroquine prevention of murine MHC-disparate acute graft-versus-host disease correlates with inhibition of splenic response to CpG oligodeoxynucleotides and alterations in T-cell cytokine production. Biology of Blood and Marrow Transplantation, 2002,08
  • [8] Kornalijnslijper J E;Daemena A J J M;van Wervena T. Bacterial growth during the early phase of infection determines the severity of experimental Escherichia coli mastitis in dairy cows. Veterinary Microbiology, 2004
  • [9] 李文波;权启镇. Immunity of CpG-DNA and the proceedings of its mechanism. 国外医学(免疫学分册), 2000,23
  • [10] Marin V;Montero Julian;Mantovani F. IL-6:A regulator of the transition from neutrophil to monocyte recruitment during in.ammation. Trends in Immunology, 2003,24
  • [11] Krieg A M;Love-Homan L;Yi A K. CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge. Journal of Immunology, 1998,161
  • [12] 张龙翔;张庭芳;李令媛. Experimental Method and Technology of Biochenmistry. 北京:高等教育出版社, 1997
  • [13] Elkins K L;Rhinehart-Jones T R;Stibitz S;Conover J S,Klinman D M. Bacterial DNA containing CpG motifs stimulates lymphocyte-dependent protection of mice against lethal infection with intracellular bacteria. Journal of Immunology, 1999,162
  • [14] Erskine R J;Bartlett P C;Tavernier S R. Recombinant bovine interleukin-2 and dry cow herapy:Efficacy to cure and prevent intramammary infections,safety and effect on gestation. Journal of Dairy Science, 1998
  • [15] Sordillo L M;Campos M;Babiuk L A. Antibacterial activity of bovine mammary gland lymphocytes following treatment with interleukin-2. Journal of Dairy Science, 1991,74
  • [16] Sordillo L M;Snider M;Hughes H. Pathological changes in bovine mammary glands following intramammary infusion of recombinant interleukin-2. Journal of Dairy Science, 1991,74
  • [17] Susantha Gomis;Lorne Babiuk;Dale L Godson. Protection of chickens against E.coli infections by DNA containing CpG-DNA motifs. Infection and Immunity, 2003,71
  • [18] Wilson D J;Herer P S;Sears P M. N-Acetyl-P-D-glucosaminidase,etiologic agent and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows. Journal of Dairy Science, 1991,74
  • [19] Winter P;Colditz L G. Immunological response of the lactating ovine udder following experimental challenge with Staphylococcus epidermidis. Veterinary Immunology and Immunopathology, 2002
查看更多︾
相似文献 查看更多>>
52.55.186.225