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用玻璃微管法冷冻猪卵母细胞
  • 摘要

    用自制的玻璃微管(glass micropipette,GMP)冷冻猪卵母细胞.以FDA染色鉴定冷冻-解冻后卵母细胞存活力,结果表明,用常规细管程序化冷冻和GMP管玻璃化冷冻猪成熟(MⅡ期)卵母细胞时,冻后存活率分别为34.5%和63.3%(P<0.05);以玻璃化冷冻比较细管和GMP管对猪MⅡ期卵母细胞冷冻效果的影响,两组冻后存活率分别为45.0%和65.9%(P<0.05);用常规细管程序化和GMP管玻璃化冷冻猪GV期卵母细胞时,冻后存活率为30.0%对59.7%,有显著性差异(P<0.05).研究表明,GMP法为一种有效的猪卵母细胞冷冻保存方法.

  • 作者

    朱良成  芮荣  杨德吉  张德福  刘东  ZHU Liang-cheng  RUI Rong  YANG De-ji  ZHANG De-fu  LIU Dong 

  • 作者单位

    南京农业大学动物医学院,南京,210095/上海市农业科学院畜牧兽医研究所,上海,201106

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    玻璃微管法  卵母细胞  冷冻保存   

参考文献
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