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CB处理和胞质去除对山羊孤雌胚体外发育的影响
  • 摘要

    为优化山羊核移植方案,实验研究了细胞松弛素B(cytochalasin-B,CB)处理和胞质去除对山羊孤雌胚体外发育的影响.将山羊MⅡ期卵母细胞用7.5μg/mL CB分别处理5、10、15、20和30 min(实验Ⅰ),处理后分别去除1/4~1/2的细胞质(实验Ⅱ),并在每个实验中设对照组(不做任何处理),孤雌激活后,用碘化丙啶和Hoechst 33258对囊胚内细胞团(ICM)细胞及滋养层(TE)细胞进行双染色,分别记录ICM和TE细胞数.结果表明,实验组Ⅰ及对照组均获得了较高的卵裂率(分别为76.83%~85.50%和86.50%)及较高的囊胚期发育率(分别为57.40%~62.20%和59.80%),囊胚细胞数及ICM细胞数在各组间无统计学差异(P>0.05);实验Ⅱ,随着去除胞质比例的增大,孤雌胚的卵裂率(75.76%~16.07%)、囊胚率(38.67%~6.67%)、囊胚细胞总数(107.15~63.67)及ICM百分率(26.32%~19.37%)呈下降趋势,超过1/3时孤雌胚的发育力显著降低(P<0.05).实验结果表明,7.5μg/mLCB在30 min内对山羊孤雌胚体外发育力无不利影响;胞质去除量控制在1/3以下对孤雌胚的发育影响不大.

  • 作者

    刘凤军  张玉玲  赵明涛  王国华  王勇胜  郑月茂  张涌  LIU Feng-jun  ZHANG Yu-ling  ZHAO Ming-tao  WANG Guo-hua  WANG Yong-sheng  ZHENG Yue-mao  ZHANG Yong 

  • 作者单位

    西北农林科技大学生物工程研究所,杨凌,712100

  • 刊期

    2006年5期 ISTIC PKU

  • 关键词

    山羊  细胞松弛素B  胞质去除  孤雌发育  双染色法 

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