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猪肥胖基因片断的克隆及不同日龄猪肥胖基因表达的差异
  • 摘要

    从杜长嘉(Duroc×Landrace×Taihu)猪的皮下脂肪中提取基因组RNA,用RT-PCR扩增肥胖基因(obese gene;ob),获得1条504bp的片断,以pGEM-T EasyVector为载体,将该基因片断克隆到大肠杆菌(Escherichia coli)中.从筛选到的阳性克隆中分离出肥胖基因,测定其序列,分析表明该片段为肥胖基因cDNA的部分序列,编码167个氨基酸组成的多肽,是成熟肽的主体部分.研究得到的基因片段与报道的猪脂肪组织中ob cDNA部分序列同源性达到99.41%,氨基酸序列同源性达到98.94%.以ob基因片段的克隆为基础,构建了优化的半定量RT-PCR法,以β-actin为内标,研究不同日龄的猪脂肪组织ob基因表达的差异,1~28日龄ob基因表达呈上升趋势,28日龄达到最高,随后,到56日龄ob基因表达又呈下降趋势.

  • 作者

    汪以真  初晓娜  黄海青  韩菲菲  WANG Yi-zhen  CHU Xiao-Na  HUANG Hai-qing  HAN Fei-Fei 

  • 作者单位

    浙江大学饲料科学研究所,杭州,310029

  • 刊期

    2005年2期 ISTIC PKU

  • 关键词

      肥胖基因  皮下脂肪  RT-PCR  基因克隆  基因表达 

参考文献
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