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马哈利樱桃PGIP基因克隆及全序列测定
  • 摘要

    以马哈利樱桃(Prunus mahaleb L.)基因组DNA为模板,用PGIP(多聚半乳糖醛酸酶抑制蛋白)基因保守序列为引物进行PCR扩增,得到长度约为1.2 kb的目的片段,将其克隆到pUCm-T载体上,进行序列测定.结果表明,目的片段全长1 192 bp,由2个外显子和1个内含子构成.外显子总长990 bp,编码330个氨基酸,与杏的PGIP基因mRNA序列、编码的氨基酸序列同源性分别达到94.1 %、96.7 %.

  • 作者

    张开春  张军科  Zhang Kaichun  Zhang Junke 

  • 作者单位

    北京市农林科学院林业果树研究所,北京香山,10093/西北农林科技大学,陕西杨陵,712100

  • 刊期

    2000年3期 ISTIC PKU

  • 关键词

    樱桃  多聚半乳糖醛酸酶抑制蛋白  基因克隆  测序 

参考文献
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