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猪生殖和呼吸综合征病毒中国分离株B13株ORF5基因在杆状病毒系统中的表达
  • 摘要

    本研究将PRRSV B13株ORF5基因克隆到杆状病毒转移载体质粒pVL1393中,构建了重组转移载体质粒pVL1393-ORF5.用该重组转移载体质粒与线性化苜蓿丫纹夜蛾核型多角体病毒(AcMNPV-SVI-G)基因组DNA(BaculoGold Linearized Baculovirus DNA)共转染草地夜蛾(Spodoptera frugiperda,sf9)细胞,得到重组病毒AcMNPV-OCC-- ORF5 .在感染了重组病毒的sf9细胞中成功地检测到分子量为25kD的ORF5基因表达产物,其能被猪抗PRRSV B13株多克隆血清特异识别.表明sf9细胞表达了所期望的产物,进而也说明基因片段的正确性.猪抗PRRSV VR-2332株多克隆血清对ORF5基因的表达产物无反应,而猪抗LV株多克隆血清则能特异识别,说明PRRSV中国分离株B13株在抗原性上更接近欧洲亚群分离株LV株的抗原性,进一步证实了PRRSV中国分离株B13株属于欧洲亚群.ORF5基因表达产物与天然蛋白分子量十分接近,说明杆状病毒表达系统能较适当的进行合成蛋白的加工和修饰.本研究的结果为PRRS基因工程疫苗的研究奠定了基础.

  • 作者

    赵耘  罗长宝  林志雄  陈茹  李树根  陈博文  刘尚高  Zhao Yun  Luo Changbao  Lin Zhixiong  Chen Ru  Li Shugen  Chen Bowen  Liu Shanggao 

  • 作者单位

    中国兽药监察所,北京,100081/广州动植物检疫局,广州,510406/中国农业大学,北京,100094

  • 刊期

    2000年3期 ISTIC PKU

  • 关键词

    PRRSV  ORF5基因  杆状病毒表达载体  表达 

参考文献
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