登录 | 注册 | 退出 | 公司首页 | 繁体中文 | 满意度调查
综合馆
五肽Pro-Gly-His-Glu-Tyr的合成及与铜(Ⅱ)离子的作用
  • 摘要

    通过固相多肽合成法合成了一个新的五肽Pro-Gly-His-Glu-Tyr,电喷雾质谱法测得其分子量与理论值一致.利用荧光光谱法研究了该五肽与金属离子的相互作用.结果表明,Cu2+对该肽具有很强的荧光猝灭效应,而其他金属离子的荧光猝灭较弱.该肽对Cu2+的这种特异性作用是由其独特的氨基酸序列所决定的.荧光光谱研究表明,Cu2+对五肽的猝灭机理为静态猝灭,五肽与Cu2+形成复合物的结合常数K为5.54×104.

  • 作者

    王召璐  李磊  李艳  李连之  WANG Zhao-lu  LI Lei  LI Yan  LI Lian-zhi 

  • 作者单位

    聊城大学化学化工学院,山东聊城,252059

  • 刊期

    2015年3期 ISTIC PKU

  • 关键词

    多肽  金属离子  Cu2+离子  荧光光谱  peptide  metal ions  Cu2+ ion  fluorescence spectra 

参考文献
  • [1] 常希俊,黄艳,贺群. 铱(Ⅳ)离子与人血丙种球蛋白的作用研究. 化学学报, 2005,3
  • [2] Malvankar P L;Shinde V M. Ion-pair extraction and determination of copper(Ⅱ) and zinc (Ⅱ) in environmental and pharmaceutical samples. ANALYST, 1991
  • [3] Muhlradt P F;Kiess M;Meyer H. Isolation,struchue elucidation,and synthesis of a macrophage stimulatory lipopeptide from mycoplasma fermentans acting at picomolar concentration. Journal of Experimental Medicine, 1997,11
  • [4] Beltramello M;Gatos M;Mancin F. A new selective fluorescence chemosensor for Cu(Ⅱ) in water. Tetrahedron Letters, 2001,52
  • [5] Lepecq J B;Paoletti C. A new fluorometric method for RNA and DNA determination. Analytical Biochemistry, 1996
  • [6] 杨曼曼;杨频;张立伟. 荧光法研究咖啡酸类药物与白蛋白的作用. 科学通报, 1999,1
  • [7] Mercer J F B. The molecular basis of copper-transport diseases. Trends in Molecular Medicine, 2001,2
  • [8] Harrison M D;Dameron C T. Molecular mechanisms of the Menkes disease protein. J Biochem.Mol Toxicol, 1999,2
  • [9] Strausak D;Mercer J F B;Dieter H H;Stremmel W. Copper in disorders with neurological symptoms:Alzheimer' s,Menkes,and Wilson diseases. BRAIN RESEARCH BULLETIN, 2001,2
  • [10] Kim J S;Quang D T. Calixarene-derived fluorescent probes. CHEMICAL REVIEWS, 2007,9
  • [11] Li Y;Li L Z;Pu X W. Synthesis of a ratiometric fluo-rescent peptide sensor for the highly selective detection of Cd2+. Bioorganic and Medicinal Chemistry Letters, 2012
  • [12] Fisk J D;Gellman S H. A parallel β-shect model system that folds in water. Journal of the American Chemical Society, 2001,2
  • [13] Harford C;Sarkar B. Amino terminal Cu(Ⅱ)-and Ni(Ⅱ)-binding (ATCUN) motif of proteins and peptide:metal binding,DNA cleavage,and other properties. Accounts of Chemical Research, 1997,3
  • [14] Fields G B;Nobel R L. Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. International Journal of Peptide and Protein Research, 1990,3
查看更多︾
相似文献 查看更多>>
3.94.202.172