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邻香草醛缩胡椒乙胺席夫碱锌(Ⅱ)配合物的研究
  • 摘要

    本文以邻香草醛缩胡椒乙胺席夫碱(L)为配体,合成一种新的席夫碱-锌(Ⅱ)双核配合物[Zn2(L)2 Cl2](1),并通过谱学分析及 X 射线单晶衍射分析对配合物1进行结构表征,通过 MTT 法测试配合物1及 L 对6种人肿瘤细胞株和人正常肝细胞株 HL-7702的体外增殖抑制活性。结果显示,配合物1 对所有肿瘤株的增殖抑制率均高于配体,但低于顺铂;对 HL-7702,其细胞毒性也远低于顺铂,且略低于配体,表现出一定的细胞毒性选择性。在分子水平上,通过荧光光谱法和凝胶电泳实验研究配合物1 及 L 与 DNA 的作用机制。结果显示,配合物1 以经典插入方式与 DNA 结合,而 L 对 DNA 的插入作用弱,表明柔性配体 L 与锌(Ⅱ)配位后,芳香平面结构刚性增强,使配合物1 对 DNA 插入作用增强,从而能够通过阻碍肿瘤细胞 DNA复制而表现显著高于 L 的抗肿瘤活性。

  • 作者

    霍红月  李仲庆  覃其品  刘延成  陈振锋  HUO Hong-yue  LI Zhong-qing  QIN Qi-pin  LIU Yan-cheng  CHEN Zhen-feng 

  • 作者单位

    广西师范大学 化学与药学学院,广西 桂林 541004; 广西师范大学 药用资源化学与药物分子工程国家重点实验室培育基地,广西 桂林 541004

  • 刊期

    2014年3期 ISTIC PKU

  • 关键词

    席夫碱  锌(Ⅱ)配合物  晶体结构  抗肿瘤活性  DNA结合  Schiff base  zinc(Ⅱ)complex  crystal structure  antitumor activity  DNA binding 

参考文献
  • [1] 胡宗球,施少敏,贺红武,丁瑜,加兵. 一维双核锌配合物[Zn2(SHSH)2(2-Me-py)2]的合成、晶体结构及理论研究. 无机化学学报, 2007,02
  • [2] 陈思园,杨扬,彭艳,刘延成. 一种新型希夫碱钯配合物的晶体结构及抗肿瘤活性研究. 广西师范大学学报(自然科学版), 2013,02
  • [3] ARGYRIOU A A,ICONOMOU G,KALOFONOS H P.Bortezomib-induced peripheral neuropathy in multiple mye-loma:a comprehensive review of the literature[J].Cancer Treat Rev,2008,34:368-377.. 2008
  • [4] MCWHINNEY S R,GOLDBERG R M,MCLEOD H L.Platinum neurotoxicity pharmacogengtics[J].Mol Cancer T-her,2009,8:10-1 6.. 2009
  • [5] POLYANSKAYA T V,KAZHDAN I,MOTLEY D M,et al.Synthesis,characterization and cytotoxicity studies of palladium(Ⅱ)-proflavine complexes[J].J Inorg Biochem,2010,104(1 1):1205-1213.. 2010
  • [6] ROSU T,PAHONTU E,PASCULESCU S,et al.Synthesis,characterization antibacterial and antiproliferative activ-ity of novel Cu(Ⅱ)and Pd(Ⅱ)complexes with 2-hydroxy-8-R-tricyclo 【7.3.1.0.2,7】 tridecane-13-one thiosemicarbazone[J].Eur J Med Chem,2010,45(4):1 627-1 634.. 2010
  • [7] LI Ming-xue,CHEN Chun-ling,ZHANG Dong,et al.Mn(Ⅱ),Co(Ⅱ)and Zn(Ⅱ)complexes with heterocyclic sub-stituted thiosemicarbazones:Synthesis,characterization,X-ray crystal structures and antitumor comparison[J].Eur J Med Chem,2010,45(7):31 69-31 77.. 2010
  • [8] MARZANO C,PELLEI M,TISATO F,et al.Copper complexes as anticancer agents[J].Anti-Cancer Agents in Me-dicinal Chemistry(Formerly Current Medicinal Chemistry-Anti-Cancer Agents),2009,9(2):185-21 1.. 2009
  • [9] JIANG Jie,TANG Xiao-liang,DOU Wei,et al.Synthesis and characterization of the ligand based on benzoxazole and its transition metal complexes:DNA-binding and antitumor activity[J].J Inorg Biochem,2010,104(5):583-591.. 2010
  • [10] WANG Rong-min,HE Nai-pu,SONG Peng-fei,et al.Preparation of low-molecular-weight chitosan derivative zinc complexes and their effect on the growth of liver cancer cells in vitro [J].Pure Appl Chem,2009,81(12):2397-2405.. 2009
  • [11] CHELLAIAN J D,JOHNSON J.Spectral characterization,electrochemical and anticancer studies on some metal(Ⅱ)complexes containing tridentate quinoxaline Schiff base[J].Spectrochim Acta A,2014,127:396-404.. 2014
  • [12] Agilent Technologies Inc.CrysAlisPro(Version 1.1 71.36.28)[CP/CD].Santa Clara,CA:Agilent Technologies Inc,2013.. 2013
  • [13] SHELDRICK G M.A short history of SHELX[J].Acta Cryst,2008,A64:1 12-122.. 2008
  • [14] DOLOMANOV O V,BOURHIS L J,GILDEA R J,et al.OLEX2:a complete structure solution,refinement and a-nalysis program[J].J Appl Cryst,2009,42:339-341.. 2009
  • [15] ADAMS H,BAILEY N A,FENTON D E,et al.Zinc(Ⅱ)complexes of tripodal ligands providing phenolate and pyridine donors:formation,structure and hydrolytic activity[J].J Chem Soc Dalton Trans,1 996:2857-2865. 1996
  • [16] STREKOWSKI L,WILSON B.Noncovalent interactions with DNA:An overview[J].Mut Res,2007,623:3-13.. 2007
  • [17] PUCCI D,BELLINI T,CRISPINI A,et al.DNA binding and cytotoxicity of fluorescent curcumin-based Zn(Ⅱ)complexes[J].Med Chem Commun,2012,3:462-468.. 2012
  • [18] DENNY W A,FERGUSON L R.Genotoxicity of non-covalent interactions:DNA intercalators[J].Mut Res,2007, 623:14-23.. 2007
  • [19] LAKOWICZ J R.Principles of fluorescence spectroscopy[M].3rd Ed.Berlin:Springer-Verlag,2006:278-282.. 2006
  • [20] CHEN Zhen-feng,LIU Yan-cheng,LIU Li-min,et al.Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine(TCM)liriodenine with metal ions,and DNA binding studies[J].Dalton Trans,2009,2:262-272.. 2009
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